Figure 8.
Immunolocalization of overexpressed Chs3p-3XHA. (A) Characteristic ER staining of strain Y604 transformed with pRS423::CHS3-3XHA after immunodetection of Chs3p-3XHA. (B) Chs3p-3XHA localization in Y604 cotransformed with pRS423::CHS3-3XHA and pRS426::CHS7. Note the dotted staining in most cells, some showing polarized staining (arrows). Some cells also show residual perinuclear staining.