Table II.
Characteristic Phenotypes of cwr6 (chs7) Mutants
| Phenotype | Strains* | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| WT | Δchs3 | cwr6 (chs7) | Δchs7 | Δchs7 (CHS7) | ||||||
| Calcofluor resistance‡ | − | + | + | + | − | |||||
| Septa enlargement§ | + | − | − | − | + | |||||
| Chitin ¶ | 100 | 6.5 | 21.6 | 13.6 | 87.6 | |||||
| CSIII ¶ | 100 | 15.8 | 28.2 | 14.6 | 103.4 | |||||
| Mating efficiency ¶ | 100 | 69.3 | ND | 65.3 | ND | |||||
| Compact ascospores‖ | No | Yes | ND | Yes | ND | |||||
*
All strains used, except the original cwr6 mutant, are isogenic derivatives of HVY374 obtained by gene replacement (Table I).
‡
Growth in buffered minimal media supplemented with 1 mg/ml Calcofluor.
§
Presence (+) or absence (−) of enlarged septa after 2 h of growth in YEPD media supplemented with 75 μg/ml Calcofluor.
¶
Numeric values represent the percentage of the values obtained for each strain compared to that of wild-type. Chitin, CSIII, and mating efficiency were determined as described in Materials and Methods.
‖
Determined by observation under phase-contrast microscope.