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. 1999 Jun 28;145(7):1497–1509. doi: 10.1083/jcb.145.7.1497

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L fibroblasts overexpressing membrane proteins lacking the ERM-binding ability. L transfectants transiently expressing wild-type E-cadherin (E-cad, a and b), E-44/20-70, a mutant E-44 lacking the juxta-membrane ERM-binding region (E-44/20-70, c and d), or E-43/KRR:NGG, a mutant E-43 bearing site-directed disrupted ERM-binding region (E-43/ KRR:NGG, e and f), were doubly stained with anti–E-cadherin mAb (a, c, and e) and anti-ERM mAb (b, d, and f). No elongated microvilli were observed. Cells were transfected by microinjection (a, b, e, and f) or by lipofection (c and d). Bar, a–f, 10 μm; insets, 2.5 μm.