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. 1999 Jun 28;145(7):1497–1509. doi: 10.1083/jcb.145.7.1497

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Microvillar elongation in CV-1 fibroblasts transiently overexpressing E-43. Cells were doubly stained with anti–E-cadherin mAb (E-43, a) and anti-ERM mAb (ERM, b). In E-43– expressing CV-1 cells, microvilli were significantly elongated by recruiting E-43 as well as ERM proteins as compared to surrounding nontransfected cells (a and b). E-43 overexpression did not appear to increase the density of microvilli. Microvillar elongation was more clearly demonstrated at higher magnification when nontransfected cells (c) and E-43–overexpressing cells (d) were stained with anti-ERM mAb. Cells were transfected by microinjection. Bars, a and b, 10 μm; c and d, 5 μm.