Figure 7.
Transient overexpression of E-43 or E-44 in MTD-1A epithelial cells. Cells were doubly stained with anti–E-cadherin mAb (a) and anti-ERM mAb (b) or with anti–E-cadherin pAb (c, e, and g) and anti-CD44 mAb (d, f, and h). Overexpression of E-43 (a–d) or E-44 (e and f) did not induce microvillar elongation (a and b), but caused exclusion of CD44 from preexisting microvilli (c–f). Overexpression of E-cadherin (g and h) did not affect the distribution of CD44. Cells were transfected by microinjection. Bar, 10 μm.