Figure 3.
Summary of sister chromatid separation/segregation defects in ipl1-2 and sli15-3 cells. The localization of TetR-GFP (small dots) and chromosomal DNA masses (shaded) were examined in wild-type (WT) (CCY1076-28B), ipl1-2 (CCY1077-6C), and sli15-3 (CCY1078-37B) cells that were incubated at 26°C (0 h) or for 2 h at 37°C. Uneven segregation of chromosomal DNA masses was indicated by shaded areas of different sizes. For each sample, 100 large-budded cells that were in early anaphase and 100 large-budded cells that were in late anaphase or early G1 were scored. No distinction was made between the mother and bud of these large-budded cells. Whether the centromere region of chromosome V (marked by TetR-GFP) present on sister chromatids appeared separated (sep.) or unseparated (unsep.) was indicated.
