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. 1999 Jun 28;145(7):1419–1433. doi: 10.1083/jcb.145.7.1419

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Distribution of HRP activity, ¹²⁵I-Trn radioactivity and synaptophysin/p38 immunoreactivity after subcellular fractionation on 5–25% Glycerol gradients. (A) PC12 cells expressing wild-type ssHRP^P-selectin were fed with ¹²⁵I-Trn (▵) for 1 h at 37°C, homogenized, and then PNS was fractionated on 5–25% Glycerol gradients to isolate SLMV. HRP activity for ssHRP^P-selectin (•) is expressed in arbitrary units corresponding to the amount of HRP activity in each fraction divided by that in the homogenate. (B) Aliquots from each fraction across the gradient were subjected to 10% SDS-PAGE and Western blotted with polyclonal antibody against p38.