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. 1999 Jun 28;145(7):1419–1433. doi: 10.1083/jcb.145.7.1419

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Targeting of HRP–P-selectin chimeras to SLMV in PC12 cells. PC12 cells expressing the HRP–P-selectin chimeras indicated in Fig. 2 were homogenized and fractionated using 5–25% Glycerol gradients to isolate SLMV. SLMV targeting indexes (SLMV-TI) were then calculated. Each bar represents the mean ± SE of 3–10 independent determinations for each chimera. Each SLMV-TI is expressed on a scale related to the WT chimera (SLMV-TI = 1) and the tail-less chimera (SLMV-TI = 0). Deviations of <0.015 are not displayed. (A) SLMV-TIs for the deletion mutants; (B) SLMV-TIs for the tetra-alanine substitutions; (C) SLMV-TIs for the point mutations.