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. 1999 Jun 28;145(7):1419–1433. doi: 10.1083/jcb.145.7.1419

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Targeting of internalized ¹²⁵I-2H11 to lysosomes. PC12 cells expressing the chimeras indicated were fed with ¹²⁵I-2H11 for 1 h at 37°C and subjected to a two-step fractionation procedure for isolation of lysosomal compartments (see Materials and Methods). A shows the distribution of NAGA (○) compared with HRP activity for ssHRP^P-selectin (▪) and tail-less ssHRP^{P-selectin763} (□). HRP activity was determined in each fraction and normalized to the total HRP activity in the homogenate. B shows the distribution of NAGA activity (○) compared with ¹²⁵I-2H11 radioactivity for ssHRP^P-selectin (filled plus signs) and for ssHRP^{P-selectin763} (empty plus signs). ¹²⁵I-2H11 radioactivity was counted in each fraction across the gradient and normalized to the total radioactivity in the homogenate. (C) the LTI for a variety of chimeras. Each bar represents the mean ± SE of four independent experiments.