Figure 1.
Cytosolic TBP binds the previously uncharacterized Kap, Kap114p. TBP-PrA and associated proteins were isolated from cytosol by IgG-Sepharose and eluted by an MgCl2 gradient, separated by SDS-PAGE, and visualized by Coomassie blue staining. The bands representing Kap114p and TBP-PrA are indicated. The positions of molecular mass standard in kilodaltons are shown.
