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. 1999 Jun 28;145(7):1369–1380. doi: 10.1083/jcb.145.7.1369

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Morphology defects in 5S RNA mutants. Single cells of wild-type (WT), YOK69, YSC14, and YOK74 strains were isolated on YPD medium and incubated at 30°C for 1 d. Developing clones were observed using a Zeiss microscope with a 10× objective lens and Nomarski DIC optics.