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. 1999 Jun 28;145(7):1341–1354. doi: 10.1083/jcb.145.7.1341

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Unfolding begins minutes after VP16 targeting and continues over several hours. Cells expressing GFP-repressor were injected with GFP-repressor-VP16. In vivo observations were made using lower magnification; after fixation, samples were optically sectioned using a high NA lens and the data deconvolved. Formation of the fibrillar (top and middle) structures takes place through an initial increase in size in the first couple of hours after microinjection followed by progressive expansion of the chromonema fibers. A similar expansion occurs for the ball structure (bottom). 8 h after microinjection, the precursor of the ball-like structure suggests a polar distribution of the EGFP-lac repressor-VP16 toward the periphery of the structure. The ball-shaped structure formed in less than 19 h after microinjection. Bars, 1 μm.