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. 1999 Jun 28;145(7):1341–1354. doi: 10.1083/jcb.145.7.1341

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VP16 targeting is accompanied by transcriptional activation. A03_1 cells were transiently transfected with DNA constructs containing EGFP-dimer lac repressor-VP16 (C), followed by lipid-mediated BrUTP delivery in intact cells and immunostaining for bromouridine (A). The two patterns superimposed showed a high signal of the RNA staining partially overlapping and adjacent to the EGFP-dimer lac repressor-VP16 signal over the A03_1 HSR (B).