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. 1999 May 17;145(4):837–849. doi: 10.1083/jcb.145.4.837

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Pak1 kinase activity affects character of movement. Pak1 expression was induced by growth in tetracycline-free DME as in Fig. 4. After attachment to fibronectin-coated plates, individual cells were tracked. Phase contrast micrographs shown were taken at 30-min intervals. (a) control cells; (b) wild-type Pak1, (c) Pak1^L83,L86, (d) Pak1^E423, (e) Pak1^R299 (the cell denoted by an arrow displayed from two to four lamellipodia throughout the course of the experiment), (f) Rac1^V12, and (g) Rac1^N17. Arrows mark the track of a single motile cell for each sample. Bar, 50 μm.