Figure 1.

H₂O₂ induces an apoptotic phenotype in yeast. DAPI-stained wild-type yeast after incubation for 200 min without (F) and with 3 mM H₂O₂ (A–E) in YEPD. TUNEL reaction after 200 min treatment of exponentially growing cells with 0 mM (G), 1 mM (H), 3 mM (I), 15 mM (J), and 180 mM H₂O₂ (K), stationary cells treated with 180 mM H₂O₂ (L), exponentially growing cells treated with 3 mM H₂O₂ plus 15 μg/ml cycloheximide (M), and exponentially growing cells treated with 15 μg/ml cycloheximide for 230 min (N). Survival (colony formation on YEPD plates, 100% corresponds to the number of plated cells) of wild-type yeast incubated with H₂O₂ in the absence (black) or presence (red) of 15 μg/ml cycloheximide for 200 min (O). Results were averaged from three experiments. Bars: 5 μm (A–F, G–N).