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. 1999 May 17;145(4):757–767. doi: 10.1083/jcb.145.4.757

Figure 4.

Figure 4

Figure 4

Figure 4

Yeast mutant KFY437 (allele cdc48 S565G) and yeast-expressing bax accumulate ROS. Rhodamine 123 fluorescence (A–F) and the corresponding phase contrast displays (G–L) after 2 h incubation with dihydrorhodamine 123. Wild-type control (A and G) and mutant KFY437 (B and H) grown at 28°C, wild-type control (C and I) and mutant KFY437 (D and J) grown at 37°C, strain WCG4bax-expressing bax (E and K), strain WCG4bax/bcl-XL expressing bax and bcl-XL (F and L). Fluorescence (M and N) and the corresponding phase contrast displays (Q and R) of wild-type control (M and Q) and mutant KFY437 (N and R) grown at 37°C after 2 h incubation with dichlorodihydrofluorescein diacetate. Fluorescence (O and P) and the corresponding phase contrast displays (S and T) of wild-type control (O and S) and mutant KFY437 (P and T) grown at 37°C after 10 min incubation with dihydroethidium. Flow cytometric analysis of wild-type control (U) and mutant KFY437 (V) after 2 h incubation with dihydrorhodamine 123.