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. 1999 May 17;145(4):795–808. doi: 10.1083/jcb.145.4.795

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Rhod-2 predominantly localizes within the mitochondria. Confocal fluorescence imaging of an adult rat cortical astrocyte coloaded with rhod-2/ AM (a) and MitoFluor Green/ AM (b), a mitochondrion-specific marker. The two images share striking similarities, suggesting a mainly mitochondrial compartmentalization of rhod-2. In the merged image (c) regions containing both rhod-2 and MitoFluor Green fluorescence appear yellow. (d) Scatter diagram representation of the colocalization. Identical images of rhod-2 and MitoFluor Green fluorescence should produce a clear diagonal line at 45°. Slight differences between the images caused irregular spots in the scatter diagram. The X axis represents the rhod-2 fluorescence signal and the Y axis the MitoFluor Green signal.