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. 1999 May 17;145(4):899–910. doi: 10.1083/jcb.145.4.899

Figure 4.

Figure 4

In situ hybridization to detect HEC-GlcNAc6ST transcripts in mouse lymph node. Sections of C56BL/6 mouse lymph node were hybridized with 35S-labeled sense or antisense riboprobes based on the clone corresponding to the mouse homologue of HEC-GlcNAc6ST. Dark field micrographs of the sections are shown. Signal is seen as bright dots. (A) Hybridization with antisense probe, whole lymph node shown. The only source of signal are HEVs, seen as distinctive high-walled vessels in the cortex of the node. (B) Hybridization with sense probe of section adjacent to that in A. (C) Higher magnification view of area indicated by arrow in A. Two large HEVs are evident.

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