Table II.
L-selectin/IgM and CD34 Staining of CHO Cells Transfected with Combinations of Sulfotransferase cDNAs
| cDNA transfected | MFI | |||||
|---|---|---|---|---|---|---|
| KSGal6ST | HEC-GlcNAc6ST | L-sel/IgM staining | CD34 staining | |||
| μg | ||||||
| 0 | 0 | 0 | 660 | |||
| 1.0 | 0 | 364 | 742 | |||
| 1.5 | 0 | 391 | 731 | |||
| 0 | 1.0 | 114 | 748 | |||
| 0 | 1.5 | 163 | 700 | |||
| 0.5 | 0.5 | 935 | 743 | |||
| 1.0 | 0.5 | 917 | 684 | |||
| 0.5 | 1.0 | 830 | 703 | |||
CHO/FTVII/C2GnT cells were cotransfected with plasmids encoding CD34 (2 μg) and each sulfotransferase singly or in combination in the indicated amounts. Data are expressed as the mean fluorescence intensity (MFI) of L-selectin/IgM staining in the L-sel–IgM+/CD34+ population with background signal (from transfectants with CD34 cDNA alone, value 139) subtracted. Also shown is the MFI for staining with a CD34 mAb (Qbend-10) for the population that was positive for CD34, as defined by staining with the class-matched control antibody.