Table 1.

Recovery of MNP is independent of divalent cations, kinase activity, and nucleotides

Sample	CEs (SpiCen300)	Asters (tubulin)
Centrosomes	50	50
KICR	50	0
KICR (extract)	50	50
KICR (extract + 5 mM EDTA)	50	50
KICR (extract + 100 mM EDTA)	50	50
KICR (extract + 5 mM DMAP)	50	50
KICR (extract + 50 mM DMAP)	50	50
KICR (extract: G-25 Spin column)	50	50

Coverslips containing centrosomes (CEs) or KICRs were treated with PEM buffer or treated high-speed extracts, washed, incubated with tubulin, fixed, and processed for immunofluorescence using antibodies to SpiCen300 and tubulin. Coverslips were then analyzed by counting 50 SpiCen300 staining CEs or KICRs and determining how many of these had corresponding asters, judged by the tubulin staining.