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. 1998 Aug 4;95(16):9307–9312. doi: 10.1073/pnas.95.16.9307

Figure 5.

Figure 5

On-line monitoring of insulin promoter-driven GFP expression (A) and c-fos promoter-driven GFP expression (B) in HIT-T15. On-line monitoring was started 20 min after the start of stimulation with glucose for rIns1GFP and with PMA for c-fosGFP. The 20-min value of GFP fluorescence was taken as 1.0. (A) On-line monitoring of individual HIT cells (n = 7) transfected with prIns1GFP and stimulated with 16.7 mM glucose for 15 min is shown as ■. Nonstimulated cells transfected with prIns1GFP (n = 10) are presented as ○. Cells transfected with pCMVGFP and stimulated with 16.7 mM glucose for 15 min are shown as ▴ (n = 10). (B) On-line monitoring of HIT cells transfected with pc-fosGFP. Cells stimulated for 15 min with 100 ng/ml PMA are shown as ■ (n = 7), and nonstimulated transfected cells are represented as ○ (n = 10). Transfected cells pretreated with 150 nM bisindolylmaleimide (BIM) and stimulated with PMA, shown as ▴ (n = 10), were monitored from minute 60 to 240 after start of stimulation. All data are shown as mean values ± SD.