Figure 4.
Inhibition of MEKK1- and NIK-induced NF-κB activation by dominant negative IKK-α or dominant negative IKK-β. HeLa cells were transfected with 3 μg of (PRDII)2CAT; 4 μg of pcDNA3-FlagMEKK1Δ, 0.1 μg of pcDNA3-FlagNIK, or 4 μg of pcDNA3; and 4 μg of pRK-FlagIKK-α (K44A), pRK-FlagIKK-β (K44A), or pcDNA3. The total DNA dose was brought up to 11 μg with pcDNA3. Cells were harvested 40 hr after transfection. CAT activities were normalized to protein concentrations of cell extracts. Shown is a representative result from three experiments.