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. 1980 Feb;84(1):29–36. doi: 10.1017/s0022172400026486

Studies on the effect of growth medium composition on the antigenicity of Mycoplasma bovis.

C J Thorns, E Boughton
PMCID: PMC2133824  PMID: 6766155

Abstract

Serum proteins adsorbed from the culture medium were detected in Mycoplasma bovis antigens, the number and type of proteins depending on the serum used in the medium. The alpha-globulins cross-reacted with alpha-globulins from different types of sera but the gamma-globulins did not. The removal of non-specific medium antibodies by absorption showed that they affected the gel diffusion and growth precipitation tests, producing cross-reactions between M. bovis and M. bovigenitalium, but that the complement fixation, tube agglutination, and growth inhibition tests were not similarly affected. The presence of serum proteins in the antigens changed their specific reactivity in all the tests. The production of antibodies to serum proteins was increased by the use of an adjuvant, but it appeared that production of specific antibodies to the mycoplasmas was not.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bradbury J. M., Jordan F. T. Studies on the adsorption of certain medium proteins to Mycoplasma gallisepticum and their influence on agglutination and haemagglutination reactions. J Hyg (Lond) 1972 Jun;70(2):267–278. doi: 10.1017/s0022172400022324. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Carroll E. J., Rollins M., Jasper D. E. The immune response of rabbits to 3 strains of Mycoplasma agalactiae var. bovis isolated from mastitic bovine udders. Cornell Vet. 1976 Apr;66(2):143–151. [PubMed] [Google Scholar]
  3. Erno H., Jurmanová K. Bovine mycoplasmas: serological studies by double immunodiffusion, growth precipitation and growth inhibition. Acta Vet Scand. 1973;14(4):524–537. [PubMed] [Google Scholar]
  4. Jordan F. T., Kulasegaram P. Non-specific antibodies in chickens inoculated intratracheally with Mycoplasma gallisepticum. J Comp Pathol. 1968 Oct;78(4):407–414. doi: 10.1016/0021-9975(68)90038-8. [DOI] [PubMed] [Google Scholar]
  5. Masover G. K., Mischak R. P., Hayflick L. Some effects of growth medium composition on the antigenicity of a T-strain mycoplasma. Infect Immun. 1975 Mar;11(3):530–539. doi: 10.1128/iai.11.3.530-539.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Morris J. A., Hussaini S. N. Characterization of the antibodies detected by the microscopic agglutination test for bovine leptospirosis. J Hyg (Lond) 1974 Dec;73(3):425–432. doi: 10.1017/s0022172400042789. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Morton H. E., Roberts R. J. Production of anti-Mycoplasma (PPLO) antibodies in rabbits. Proc Soc Exp Biol Med. 1967 Jun;125(2):538–543. doi: 10.3181/00379727-125-32140. [DOI] [PubMed] [Google Scholar]
  8. Smith S. C., Dunlop W. R., Strout R. G. Effect of culture medium on antigenic structure of mycoplasma. Avian Dis. 1966 May;10(2):173–176. [PubMed] [Google Scholar]
  9. Thorns C. J., Boughton E. Studies on film production and its specific inhibition, with special reference to Mycoplasma bovis (M. agalactiae var. bovis). Zentralbl Veterinarmed B. 1978 Oct;25(8):657–667. doi: 10.1111/j.1439-0450.1978.tb00775.x. [DOI] [PubMed] [Google Scholar]
  10. Thorns C. J. Identification of the products of the 'film spots' reaction of Mycoplasma bovis and Mycoplasma bovigenitalium. J Biol Stand. 1978;6(2):127–132. doi: 10.1016/s0092-1157(78)80044-4. [DOI] [PubMed] [Google Scholar]

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