Skip to main content
The Journal of Hygiene logoLink to The Journal of Hygiene
. 1981 Feb;86(1):79–85. doi: 10.1017/s0022172400068765

Detection and quantification of IgM, IgA, IgG1 and IgG2 antibodies against foot-and-mouth disease virus from bovine sera using an enzyme-linked immunosorbent assay.

E M Abu Elzein, J R Crowther
PMCID: PMC2134065  PMID: 6257779

Abstract

A simple solid-phase enzyme immunoassay is described for the detection of antibody classes showing activity against foot-and-mouth disease (FMD) virus in bovine sera. The assay achieves a preliminary separation of the specific class of antibody from other serum proteins through immuno-adsorption to class-specific immunoglobulin-coated wells of micro-titre plates. The specific antibody is reacted with FMD virus, which is then detected by an enzyme-labelled anti virus IgG.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abu Elzein E. M., Crowther J. R. Enzyme-labelled immunosorbent assay techniques in foot-and-mouth disease virus research. J Hyg (Lond) 1978 Jun;80(3):391–399. doi: 10.1017/s0022172400024840. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Avrameas S. Coupling of enzymes to proteins with glutaraldehyde. Use of the conjugates for the detection of antigens and antibodies. Immunochemistry. 1969 Jan;6(1):43–52. doi: 10.1016/0019-2791(69)90177-3. [DOI] [PubMed] [Google Scholar]
  3. BROWN F., CARTWRIGHT B., NEWMAN J. F. FURTHER STUDIES OF THE EARLY ANTIBODY IN THE SEA OF CATTLE AND GUINEA PIGS INFECTED WITH FOOT-AND-MOUTH DISEASE VIRUS. J Immunol. 1964 Sep;93:397–402. [PubMed] [Google Scholar]
  4. BROWN F., CARTWRIGHT B. PURIFICATION OF RADIOACTIVE FOOT-AND-MOUTH DISEASE VIRUS. Nature. 1963 Sep 21;199:1168–1170. doi: 10.1038/1991168a0. [DOI] [PubMed] [Google Scholar]
  5. Sloan G. J., Butler J. E. Evaluation of enzyme-linked immunosorbent assay for quantitation by subclass for bovine antibodies. Am J Vet Res. 1978 Jun;39(6):935–941. [PubMed] [Google Scholar]

Articles from The Journal of Hygiene are provided here courtesy of Cambridge University Press

RESOURCES