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. 1997 Feb 10;136(3):621–631. doi: 10.1083/jcb.136.3.621

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The small Ank1 isoforms in skeletal muscle are unaffected by the nb/nb mutation. Cross sections of diaphragms from wild-type (A, C, and E) and nb/nb (B, D, and F) mice were labeled with nonimmune rabbit serum (A and B), p65 antibodies to the spectrin-binding domain of ankyrin 1 (C and D), and p6 antibodies to the small ankyrins (E and F), followed by rhodamineconjugated secondary antibodies. Samples were taken from unperfused animals, so erythrocytes remaining in the capillaries could react with the antibodies if they contained the appropriate antigens. The results show that skeletal muscle fibers in the nb/nb mouse selectively lack the 210-kD Ank1 at the sarcolemma but are not deficient in the small myoplasmic forms. Bars, 20 μm.