Table II.

Specific c2-10 DNA-binding Activity Is Elevated in an Abp1p-overexpressing Strain

Strain/Extract		Volume		Total Protein		Binding Units		Specific Activity		Enrichment‡
		ml		mg		U*		U/μg
WT/Low Salt		13.1		86.2		9,630		0.11		1.0
Abp1pOE/Low Salt		13.4		102.9		30,010		0.29		2.7
WT/0.4 M KCl		14.4		6.1		8,130		1.33		12.1
Abp1pOE/0.4 M KCl		14.6		8.8		15,960		1.81		16.5

Extract was prepared from a 2-liter culture of wild-type (WT) or Abp1p-overexpressing cells.  

^* 1 U binds 1 fmol c2-10 DNA in a gel mobility shift assay.  

^‡ Normalized to the specific activity of low-salt extract from WT cells.