Figure 11.

Nuclear channels are detectable in live cells and correspond to the Con A–labeled structures seen in fixed cells. a shows a single plane from the dataset for two DiOC₆ labeled live cells; e shows the dihydroethidium label in the same plane. b and f are from data collected 20 min later. c shows the loss of the DiOC₆ signal at the end of methanol fixation, while g shows that the cells remain intact and the dihydroethidium label persists, although reduced in intensity. d shows the same cells and plane after 20 min of Con A labeling. h–j show sequential rotations of reconstructions of DiOC₆ labeled cells, and underneath in k–m, respectively, the same cell is shown after Con A labeling. Cutaway views of a reconstructed DiOC₆ ⁻ labeled HeLa nucleus from a separate experiment are shown at three time points in n–p. Note that the morphology of the larger left hand tube alters over the 35-min period, and the smaller channel shows a progressively increasing separation from the large channel. Bars: (a–m) 25 μm; (n–p) 2 μm.