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. 1997 Feb 10;136(3):531–544. doi: 10.1083/jcb.136.3.531

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Intranuclear PDI immunoreactivity co-localizes with Con-A–labeled channels. The figure shows separate eight-bit grey scale images collected from a triple labeled NRK cell using a cooled CCD camera. A shows the distribution of the PDI signal in the fluorescein channel. B shows the Con A signal in the rhodamine channel, and C shows the nucleic acid labeled with DAPI. D is a 24-bit merge of the first three channels. The arrowhead points to an intranuclear structure that can be followed through a number of focal planes in which PDI and Con A reactivity colocalize.