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. 1997 Feb 10;136(3):583–595. doi: 10.1083/jcb.136.3.583

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Turnover of αβ_18–27 in K562 cells. The cells were iodinated, washed, and incubated in chase medium. At the indicated times, cells were harvested and lysed. αβ_18–27 chimera (mol wt ∼55 kD) and transferrin receptor (mol wt ∼90 kD) were immunoprecipitated from the lysates with 7G7B6 anti-IL2Rα and OKT9 anti-transferrin receptor mAb, and separated by SDSPAGE (inset). Radioactivity in the bands was quantitated by phosphorimager analysis. The ratio of αβ_18–27 chimera to transferrin receptor was calculated at each time point. The values in the graph represent the percentage of this ratio at different times relative to that at time zero.