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. 1997 Feb 10;136(3):583–595. doi: 10.1083/jcb.136.3.583

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Internalization of 7G7B6 antibody in cells transfected with α or α_Y. (a) HeLa cells transiently transfected with α (A) or α_Y (B) were incubated for 30 min with 7G7B6 mAb. After fixation and permeabilization, the antibody was revealed using Texas red–labeled antimurine IgG2a antibody. Magnification: 630. (b) Kinetics of ¹²⁵I-labeled 7G7B6 mAb internalization in K562 cells stably transfected with α (○) or α_Y (•). The antibody was added to the cells at 37°C. After further incubation for the indicated times, cells were cooled to 4°C, and the amount of internalized antibody was measured. One representative experiment out of three is shown. Error bars indicate standard deviations for averages of three or more experiments.

Internalization of 7G7B6 antibody in cells transfected with α or α_Y. (a) HeLa cells transiently transfected with α (A) or α_Y (B) were incubated for 30 min with 7G7B6 mAb. After fixation and permeabilization, the antibody was revealed using Texas red–labeled antimurine IgG2a antibody. Magnification: 630. (b) Kinetics of ¹²⁵I-labeled 7G7B6 mAb internalization in K562 cells stably transfected with α (○) or α_Y (•). The antibody was added to the cells at 37°C. After further incubation for the indicated times, cells were cooled to 4°C, and the amount of internalized antibody was measured. One representative experiment out of three is shown. Error bars indicate standard deviations for averages of three or more experiments.