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. 1997 Feb 10;136(3):649–658. doi: 10.1083/jcb.136.3.649

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Interaction of Bee1p with Sla1 and actin as detected by immunoprecipitation. RLY160 extract preparation and immunoprecipitation using either mouse anti-myc antibody (Evan et al., 1985) (a) or rabbit anti-Sla1p antibody (a gift from D. Drubin) (b) were carried out as described in Materials and Methods. Immunoblot analysis of the immunoprecipitated proteins was carried out using antibodies against Sla1p, myc, Bem1p (Pringle et al., 1995), actin (Boehringer Mannheim Corp., Indianapolis, IN), and Cof1p (Moon et al., 1993). Lane 1, extract before immunoprecipitation; lane 2, eluate of the control beads (see Materials and Methods); lane 3, eluate of anti-myc beads (a) or eluate of antiSla1 beads (b).