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. 1997 Feb 10;136(3):597–607. doi: 10.1083/jcb.136.3.597

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Exocytosis of one trichocyst gives rise to a single current event. Consecutive half frames (20 ms apart) before (A) and after (B and C) exocytosis of a single trichocyst (circle) from a cell (c) attached to a holding pipette (hp). (D) Simultaneously recorded outward current signal, which is shown as inset in (A–C) (see Materials and Methods). The current event corresponding to the single trichocyst is marked by the arrow. It has a charge of 0.6 pC and is amplified in (D). Preceding large currents correspond to release of trichocysts that are already outside the viewing area. At the end of recording, trichocysts can be identified and counted much more clearly after they have settled (not shown). Data was obtained by extracellularly recorded outward currents after application of a submaximal dose of AED.

Exocytosis of one trichocyst gives rise to a single current event. Consecutive half frames (20 ms apart) before (A) and after (B and C) exocytosis of a single trichocyst (circle) from a cell (c) attached to a holding pipette (hp). (D) Simultaneously recorded outward current signal, which is shown as inset in (A–C) (see Materials and Methods). The current event corresponding to the single trichocyst is marked by the arrow. It has a charge of 0.6 pC and is amplified in (D). Preceding large currents correspond to release of trichocysts that are already outside the viewing area. At the end of recording, trichocysts can be identified and counted much more clearly after they have settled (not shown). Data was obtained by extracellularly recorded outward currents after application of a submaximal dose of AED.