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. 1997 Feb 10;136(3):597–607. doi: 10.1083/jcb.136.3.597

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Increasing [Ca²⁺]_e from 0.1 to 10 mM causes a rapid cortical Ca²⁺ transient, but no exocytosis. A Fura red–loaded cell was analyzed by the double wavelength method, after application of 10 mM [Ca²⁺]_e (no AED) at the arrowhead at t_o. [Ca²⁺]_i at this site shows a rapid transient increase at 2 s, reaching ∼1 μM at 8 s and returning to almost resting levels after 45 s. Phase contrast analysis at the end of the experiment (lower left) shows a full set of trichocysts in the cell, ∼5 μm long, at the cell membrane, none having been expelled. Bar, 20 μm.