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. 1997 Feb 10;136(3):609–620. doi: 10.1083/jcb.136.3.609

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The T. cruzi oligopeptidase B is localized in the cytoplasm of the parasites. Ultrathin cryosections of aldehyde-fixed T. cruzi were labeled with affinitypurified guinea pig anti–oligopeptidase B IgG followed by protein A–10 nm gold. The subcellular localization of oligopeptidase B in trypomastigotes (A) is similar to that of amastigotes (B). No labeling is detected in the nucleus (N), kinetoplast (K), mitochondrion (M), Golgi complex (G), on the parasite surface (S), or in the flagellar pocket (FP). Bar, 0.5 μm.