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. 1997 Feb 10;136(3):659–668. doi: 10.1083/jcb.136.3.659

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A shows a Northern blot analysis designed to detect the presence of the 3.6-kb CHO1 message in neuroblastoma cultures. Messenger RNA from cultures treated with either retinoic acid (RA), db cAMP, or neither reagent were analyzed. Detectable levels of CHO1 message were found under all three conditions. The RA condition is shown here. B and C show immunofluorescence images of mitotic neuroblastoma cells immunostained for tubulin (red) and CHO1/MKLP1 (green). B shows a cell in anaphase, with CHO1/MKLP1 staining concentrated in the midzone. C shows a cell in telophase, with CHO1/ MKLP1 staining concentrated in the midbody. Diffuse staining for CHO1/MKLP1 is also present throughout the cells. Bar, 5 μm.