Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 1997 Feb 10;136(3):659–668. doi: 10.1083/jcb.136.3.659

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Quantitative analyses on the levels of CHO1/MKLP1 (left two panels), and the levels of tubulin, dynein, and MAP2 (right three panels) in cultures treated with antisense and sense oligonucleotides. Protein levels were determined using quantitative immunofluorescence microscopy, and were expressed in arbitrary fluorescence units (AFU). Only process-bearing cells were scored in these analyses, and 100 cells were analyzed for each experimental condition and time point. Standard deviations were generally low, on the order of 15 AFU, and could not be included on the graph because the error bars would be short and blur together. Both AS1 and AS2 treatments resulted in diminished staining for CHO1/MKLP1 in a dose- and time-dependent manner. Sense controls did not affect CHO1/ MKLP1 levels. Neither antisense nor sense oligonucleotides resulted in diminutions in the levels of β-tubulin, dynein, or MAP2.