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. 2008 Jan;18(1):123–136. doi: 10.1101/gr.6940108

Figure 3.

Figure 3.

Rpd3(HDAC1) and HDAC6 modulate mitochondrial functions in vivo. (A) The CS activity in S2 cells transfected with Rpd3 and HDAC6 RNAi (n = 6). The lacZ RNAi acts as a negative control and Citrate synthase RNAi as a positive control. P-value was calculated against lacZ RNAi control group. (B) Rpd3 protein was highly reduced in the transgenic Rpd3 RNAi flies as shown by Western blot. Lysates from the Rpd3 RNAi flies and control siblings were blotted with the antibody against Rpd3 and Tubulin. The Tubulin levels were used as loading controls. (C) HDAC6 protein was highly reduced in the transgenic HDAC6 RNAi flies as shown by Western blot. Lysates from the transgenic HDAC6 RNAi and control siblings were blotted with the antibodies against HDAC6 and Tubulin. The Tubulin levels were used as loading controls. (D) The CS activity in the transgenic Rpd3 RNAi flies and control siblings (n = 8). (E) The CS activity in the transgenic HDAC6 RNAi flies and control siblings (n = 8). (F) The COX activity in the transgenic Rpd3 RNAi flies and control siblings (n = 8). (G) The COX activity in the transgenic HDAC6 RNAi flies and control siblings (n = 8). *P < 0.05, **P < 0.01, ***P < 0.001. Error bars indicate the standard errors.