Figure 1.

Three techniques support a CNV in C57BL/6J × BALB/cJ first generation offspring. (A) An Illumina platform SNP genotyping assay reveals two classes of heterozygote F₁ animals that differ in their ratio of C57BL/6J allele intensity compared to BALB/cJ allele intensity at SNP rs30920120. (B) A real-time PCR assay was used to confirm the two classes of heterozygote F₁ animals detected in the SNP genotyping assay shown in panel A. (C) Sequencing of SNP rs30920120 confirms an altered parental ratio in two representative individuals from each of the two heterozygote F₁ genotype classes.