Fig 2.

Protein carbonyls and 4-hydroxy-2-nonenals levels in plasma and lymphocytes from diabetic patients. Plasma and lymphocytes samples from type 2 diabetic patients with nephropathy (black columns) and age-matched controls (white columns) were assayed for protein carbonyls (DNPH) and 4-hydroxy-2-nonenals (HNE) by Western blot, as described in Materials and Methods. Values are expressed as mean ± standard error of mean of 3 independent analyses on 16–21 patients per group. *P < 0.05 and **P < 0.01 vs controls