Fig 1.

Hsp27 transcription from the allele associated with the EP(3)3583 element. Reverse transcriptase–polymerase chain reaction (RT-PCR) amplified from a pair of primers derived from the hsp27 transcript, Phsp27-a and Phsp27-b, are shown. Three cDNA dilutions were 1:6, 1:3, and 1:1. Optimized PCR conditions were allowed for 28 cycles to ensure DNA amplification within the exponential range. RNA was extracted from young adult flies without or with heat shock pretreatment for 1 h at 37°C and recovery for 30 min at 25°C. As positive control for cDNA synthesis, amplification of a Drosophila actin cDNA with a pair of primers, Pactin-a and Pactin-b, also was shown. Wild type control was no heat shock (Lane 1– 3) and heat shock (Lanes 7–9). The allele associated with the EP(3)3583 element was no heat shock (Lanes 4–6) and heat shock (Lanes 10–12)