Fig 2.

The hsp27^−0.7 null allele. (A) A schematic drawing of the genomic DNA flanking the EP(3)3583 element. The relative positions of the Hsp27 transcript and the deletion in the hsp27^−0.7 allele are shown. The breakpoints of the deletion (707 basepair, from +179 to +885) are separated by an insertion of 3 nucleotides (GAA, underlined). (B) Amplification of genomic DNA by polymerase chain reaction (PCR). With the Pp31 and Phsp27-a primer pair a fragment of ∼1.1 bp was produced from the starting chromosome with EP(3)3583 (control, Lane 1), whereas a fragment of ∼0.4 kb was produced from the hsp27^−0.7 allele (Lane 2). With the Pp31 and Pg-R primer pair a fragment of ∼250 bp was produced from the starting chromosome with EP(3)3583 (control, Lane 3) and from the hsp27^−0.7 allele (Lane 4)