Figure 2.

Differential effects of anti-xNup98 antibodies on transport of in vivo synthesized RNA polymerase II and III transcripts. A mixture of DNA templates encoding U4 snRNA, 5S rRNA, and tRNA^Tyr was injected into oocyte nuclei together with buffer (Control) or monospecific antibodies against nucleoporins xNup98 (α-98) or xNup200 (α200). 2 h later, α[³²P]GTP (∼1.0 μCi/oocyte) was injected into the cytoplasms of all oocytes. After 4 (A) or 21 h of labeling (B), oocytes (3–6/timepoint) were manually dissected, and the intracellular distributions of the newly made RNAs were determined by analyses of 0.5 oocyte equivalents of the nuclear (N) and cytoplasmic (C) RNAs in 8% denaturing polyacrylamide gels. The RNAs made from the injected genes are indicated. Endogenous oocyte RNAs made in the absence of injected genes (No DNA) are shown in B, lanes 7 and 8. The level of 5S DNA templates was ∼10-fold higher in A than in B.