Figure 6.

Lack of effect of anti-xNup98 antibodies on import of karyophilic proteins. Normal IgG (IgG), anti-xNup98 (α-98), or WGA was injected into oocyte nuclei (IgG, α-98) or cytoplasms (WGA). 2 h later, 0.25 oocyte equivalents of ³⁵S-labeled total soluble nuclear proteins were injected into the cytoplasm of all oocytes. After 24 h incubation, oocytes were manually dissected, and total nuclear (N) and cytoplasmic (C) extracts were prepared from pools of 3–5 oocytes. 0.5 oocyte equivalents of the extracts were analyzed on a 10% polyacrylamide gel containing SDS and ³⁵S-labeled proteins were detected by autoradiography. Arrows indicate the strongly labeled karyophilic proteins N1/N2 (Dabauvalle and Francke, 1982). Lines indicate the positions of molecular weight markers of 200, 97, and 66 kD.