Table II.
Plasmids Used in This Study
| Plasmid | Vector | MFA1 genotype | Yeast marker | Source | ||||
|---|---|---|---|---|---|---|---|---|
| pDH6 | pRS306 | mfa1-Δ1 | URA3 | D. Hagen and G. Sprague | ||||
| pDH9 | pRS306 | mfa2-Δ1 | URA3 | D. Hagen and G. Sprague | ||||
| pMC1871 | pBR322-LacZ | − | − | Casadaban et al. 1983 | ||||
| pRS316 | CEN | − | URA3 | Sikorski and Hieter, 1989 | ||||
| pRS314 | CEN | − | TRP1 | Sikorski and Hieter, 1989 | ||||
| pRS424 | 2μ | − | TRP1 | Christianson et al., 1992 | ||||
| pSM18 | pUC18 | MFA1 | − | Michaelis and Herskowitz, 1988 | ||||
| pSM29 | pUC18 | MFA2 | − | Michaelis and Herskowitz, 1988 | ||||
| pSM38 | pUC18 | mfa2-Δ2::lacZ | − | This study | ||||
| pSM32 | pUC18 | mfa1-Δ1 | − | Michaelis and Herskowitz, 1988 | ||||
| pSM118 | pGEM1 | SP6-MFA1 | − | This study | ||||
| pSM217 | 2μ | − | URA3 | This study | ||||
| pSM219 | 2μ | MFA1 | URA3 | This study | ||||
| pSM233 | CEN | MFA1 | URA3 | This study | ||||
| pSM257 | 2μ | mfa1-C33S | URA3 | This study | ||||
| pSM258 | 2μ | mfa1-ΔVIA | URA3 | This study | ||||
| pSM463 | 2μ | MFA1 | TRP1 | This study | ||||
| pSM464 | CEN | MFA1 | TRP1 | This study | ||||
| pSM490 | 2μ | mfa1-I23M | URA3 | This study | ||||
| pSM628 | 2μ | MFA2 | URA3 | This study | ||||
| pSM630 | CEN | MFA2 | URA3 | This study |