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Visible clustering of vacuoles in the early reaction phase depends on Ypt7p. (A) Fusion reactions for microscopic analysis were performed as in Fig. 4. Samples were incubated for 15 min at 27°C in the presence of buffer, Gdi1p (100 μg/ ml), or Fab fragments against Ypt7p (125 μg/ml) or carboxypeptidase Y (Control-Fab; 125 μg/ml). (B) Quantitation of the data in A was performed as in Fig. 3.
