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. 1997 Jan 27;136(2):287–297. doi: 10.1083/jcb.136.2.287

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Redistribution of A-ALP, (F/A)A-ALP, and RS-ALP upon induction of synthesis of Vps27p in vps27 cells. NBY67 (vps27) cells harboring either pSN55 (A-ALP), pSN100 ((F/A)A-ALP), or pSN97 (RS-ALP) and pHY5 (VPS27 under GAL1 control) (+) or empty vector (−) were grown in synthetic media containing 2% raffinose. At time t = 0, galactose was added to 2%, and cells were allowed to grow for an additional 90 min. At times (A) t = 0 (− galactose) and (B) t = 90 min (+ galactose) (also see Fig. 4), cells were fixed and prepared for immunofluorescence using anti-ALP antibodies in combination with biotin-linked secondary antibody and streptavidin-conjugated FITC, as well as anti-Vph1p antibodies in combination with rhodamine-conjugated goat anti–rabbit IgG (H + L).