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. 1997 Jan 27;136(2):319–330. doi: 10.1083/jcb.136.2.319

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Distribution of endotubin staining in transfected NRK cells. There is little expression of endotubin on the cell surface, as endotubin staining is not detected on nonpermeabilized cells (A), even though intracellular expression is high (B). In C and D, and E, NRK cells transfected with endotubin cDNA were double labeled with antibodies against endotubin (C) and lgp120 (D). The merged image (E) shows the distinct staining patterns, although there may be some colocalization in the perinuclear region (arrows). N, nucleus. Bar equals 5 μm.