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Top: Image plane within a three-dimensional image stack of MC3T3-E1 cell (red) and 1μm diameter sulfate coated fluorescent beads (green) obtained using confocal laser scanning microscopy. Bottom: Representative cross section from image stack. Outlined in the white boxes are three beads with various degrees of internalization. Note that some of the beads look smaller than others because they are slightly out of the cross sectional focal plane.
