Figure 6.

Cytokine production by LPS-stimulated BMM from IKK2^fl and IKK2^del mice. (a) IKK2^fl and (b) IKK2^del macrophages were left untreated (white) or stimulated with LPS (gray). TNF production was detected by intracellular cytokine staining and analyzed by FACS. (c) IKK2^fl and IKK2^del macrophages were stimulated with LPS. TNF production was detected by intracellular cytokine staining and analyzed by FACS. Results are representative for at least two experiments. (d) IKK2^fl and IKK2^del macrophages were stimulated overnight with LPS. Cytokines were measured in the supernatants. Shown are the changes in cytokine production by IKK2^del macrophages as compared with IKK2^fl macrophages. Results are the average of five to eight experiments. Error bars indicate SEM. *P < 0.01; ^#P < 0.01 by paired t test.