Figure 7.

IL-6 production and effects of IL-10. (a) IKK2^fl and IKK2^del macrophages were stimulated with LPS for indicated times. IL-6 was measured in the supernatants. (b) IKK2^fl and IKK2^del macrophages were stimulated with LPS for 24 hours in combination with indicated doses of recombinant mouse IL-10. IL-6 was measured in the supernatants. (c) IKK2^fl and IKK2^del macrophages were stimulated with LPS for 24 hours, in the absence or presence of blocking anti-IL-10 antibody or IgG control. IL-6 was measured in the supernatants. Figures are representative for two experiments. Error bars indicate SEM. *P < 0.05, **P < 0.01.